Open-access workflows

Protocol Hub

Step-by-step guides for complex preclinical imaging workflows. Validated protocols below are derived from NeuroSimplicity peer-reviewed publications — with video walkthroughs on our YouTube channel. Deployment and module-specific setup guides remain available to licensed users in the portal documentation.

Video walkthroughs

Watch the iterative micro-CT and neurovascular workflow on @NeuroSimplicity.

View channel

Validated by NeuroSimplicity

Cell Reports Methods · 2021Validated by NeuroSimplicity

Methods paper

Non-invasive in situ Visualization of the Murine Cranial Vasculature

Methods validation and interactive workflow overview for the iterative micro-CT pipeline.

View workflow overview

STAR Protocols · 2023Validated by NeuroSimplicity

Bench protocol

Visualization of murine in situ neurovascular interfaces

Step-by-step bench guide with timing, reagents, and QC — companion to the 2021 methods paper.

View bench guide

Both publications describe the same iterative micro-CT workflow — the 2021 methods paper validates the approach; the 2023 STAR Protocol is the step-by-step bench guide.

Curated third-party protocols

Additional published protocols for light sheet microscopy, fluorescence imaging, and spatial omics workflows will be added here with clear attribution. Third-party protocols are not validated by NeuroSimplicity and will carry an explicit disclaimer.