The Cell Reports Methods (2021) and STAR Protocols (2023) workflows — polymer casting, decalcification, PTA contrast, iterative scans — are validated with NeuroSimplicity for deformable registration across volumes in native sample space.
Used in Nature publications for dural sinuses, venolymphatic hubs, dural-associated lymphoid tissues (DALT), and skull–brain–meningeal immune interface mapping by NIH collaborators.